Cyanide Metabollism by Bacillus megaterium * ( Received for publication
نویسندگان
چکیده
Cyanide disappears from a medium supporting actively growing cells of Bacillus megaterium, a bacterium isolated from a cyanide enrichment medium containing Fargo clay soil. Whole cells of this organism convert K14CN to asparagine-*4C, aspartic acid-14C and 14C02. Besides cyanide, serine l4C and /3-cyanoalanine-14C are effectively converted by whole cells of this organism to asparagine-14C. An analysis of asparagine isolated in an experiment in which whole cells were administered K13C15N and serine-14C shows that the nitrogen and carbon of the amide group is derived from cyanide. /3-Cyanoalanine-14C was isolated and identified as a product of this experiment. These results as well as results of analyses of the carbon skeletons of P-cyanoalanine-14C and asparagine-14C from this and other experiments collectively support the hypothesis that a metabolic pathway in this organism begins with the condensation of serine and cyanide to form P-cyanoalanine which in turn is hydrolyzed to asparagine and then to aspartic acid. Furthermore cell-free extracts catalyze the conversion of K14CN and serine to asparagine-1% and aspartic acid-14C as well as the conversion of asparagine14C to aspartic acid-14C.
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